Functionalized nanoparticles as new tools for bioanalysis

CHED 309

Jacob Berger,, Department of Physics and Department of Biology, Yeshiva University, New York, NY 10033 and Duncan Graham,, Department of Pure and Applied Chemistry, University of Strathclyde, Thomas Graham Building, 295 Cathedral Street, Glasgow, G1 1XL, United Kingdom.
Metallic nanoparticles can be used as basic materials for a wide variety of purposes including building blocks for nanoassemblies, substrates for enhanced spectroscopies such as fluorescence and Raman, and as labels for biomolecules. Here we report how silver and gold nanoparticles can be functionalized with specific biomolecular probes to indicate the molecular recognition of a target molecule. Examples of this approach include DNA hybridization to switch on surface enhanced resonance Raman scattering (SERRS) when a specific target sequence is present, recognition of specific proteins by aptamer functionalised nanoparticles through surface plasmon resonance or SERRS, and the use of nanoparticles functionalized with antibodies to provide a new type of immunoassay. These examples indicate how nanoparticles can be used to provide highly sensitive and informative data from a variety of biological systems when used with optical spectroscopy.

Undergraduate Research Poster Session: Physical Chemistry
2:30 PM-4:30 PM, Monday, August 17, 2009 Walter E. Washington Convention Center -- Hall D, Poster

Division of Chemical Education

The 238th ACS National Meeting, Washington, DC, August 16-20, 2009