Quartz binding peptides as molecular linkers for co-assembling nanoentities on multifunctional micropatterned substrates


Turgay Kacar, kacart@u.washington.edu1, John Ray, jpr07@u.washington.edu1, Mustafa Gungormus, musgun@u.washington.edu2, Ersin Emre Oren, eeoren@u.washington.edu3, Candan Tamerler, candan@u.washington.edu4, and Mehmet Sarikaya, sarikaya@u.washington.edu2. (1) Materials Science and Engineering, University of Washington, GEMSEC, Roberts Hall, Box: 352120, Seattle, WA 98195, (2) GEMSEC, Department of Materials Science and Engineering, University of Washington, 302C Roberts Hall, Box 352120, Seattle, WA 98195-2120, (3) University of Washington, Seattle, WA 98195, (4) MOBGAM and Molecular Biology-Genetics, Istanbul Technical University, ITU Ayazaga Kampusu Fen Edebiyat Fakultesi, Istanbul, 34469, Turkey
Protein microarray technologies, used in proteomics and clinical assays, require efficient patterning of biomolecules on selected substrates. Immobilization and patterning of biomolecules mostly require covalent attachments of target molecule to solid substrates through chemical reagents such as alkanethiols, aminoalkylalkoxysilanes, etc.. In the case of the attachment to the quartz surface, we propose to use quartz binding peptide sequence (QBP1), instead of silane derivatives. This sequence was de novo designed based on the sequence similarity data of the string binding peptides followed by a bioinformatics similarity analysis. In this study, QBP1 is used as both ink and linker for micro-contact printing followed by directed self-assembly of co-immobilization of streptavidin-coated quantum dots and fluorescein molecule. Directed assembly of the dots is carried out following micro-contact printing of biotinylated QBP1 on a quartz surface. The remaining “untouched” regions are occupied by the assembly of fluorescein directed to bare silica regions on the patterned where QBP1 was used as the molecular linker. The patterned substrates were then visualized by fluorescence microscope. The developed procedure have potential implications in novel simultaneous co-assembly nano- and molecular functional entities.