Bioresorbable hyaluronic acid hydrogels for tissue engineering applications


Sidi A Bencherif1, Abiraman Srinivasan, abiraman@andrew.cmu.edu2, Angela Jiang, ajiang@andrew.cmu.edu1, Jeffrey O Hollinger, hollinge@andrew.cmu.edu2, Krzysztof Matyjaszewski1, and Newell R Washburn, washburn@andrew.cmu.edu3. (1) Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, (2) Bone Tissue Engineering Center, Carnegie Mellon University, Pittsburgh, PA 15213, (3) Departments of Chemistry and Biomedical Engineering, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213
In this work, biologically active and degradable hyaluronic acid was used to prepare flexible hydrogels with various degrees of cross-links to tune gel degradation rate. RGD-modified hyaluronic acid-based hydrogels promoted cell proliferation, differentiation, and mineralization of preosteoblast MC3T3-E4 cells within 4 weeks of incubation when cultured in osteogenic media (OS+). An investigation into degradation profiles of these gels has shown that highly cross-linked hydrogels were more suitable to allow mineralization of cells as they degraded after a month when incubated in enzymatic solutions. The objective is to design a cell-adhesive scaffold with tunable degradation profiles that would balance the need to maintain structural support for cellular proliferation, extracellular matrix (ECM) secretion, and mineralization in response to HA-based hydrogels, with the need for polymer disintegration to leave room for new tissue growth.