BIOT 51 |
| The design and optimization of two novel Protein A affinity resins will be described. Critical design parameters such as the pore size, particle size and ligand density will be compared for high capacity resins based on separate approaches, one involving an agarose base matrix and another involving a controlled pore glass base matrix. A novel affinity ligand coupling method, so called “associative coupling”, was implemented which allows for increased IgG binding capacity. This coupling method also provides controlled and/or reduced non-specific binding. For both resins IgG saturation binding capacities greater than 70g/L can be achieved. However, the resulting dynamic capacity is very different. Chromatography performance for antibody capture will be discussed for both novel resins. |
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Downstream Processing: Advances in Chromatography
1:30 PM-4:25 PM, Sunday, August 17, 2008 Philadelphia Marriott -- Grand Blrm Salon I/J, Oral
Division of Biochemical Technology |