Development of a device for prion reduction based on affinity ligand

I&EC 64

Patrick V. Gurgel1, M. Omon Herigstad, moherigs@ncsu.edu2, Yong Zheng3, Luisa Gregori4, Julia Lathrop5, Robert Rohwer, rrohwer@umaryland.edu6, Peter Edwardson3, and Ruben G Carbonell, ruben@ncsu.edu2. (1) Department of Chemical and Biomolecular Engineering, ProMetic Life Sciences and NC State University, 8168 chemin Montview, 911 Partners Way, Mont-Royal, QC H4P 2L7, Canada, (2) Department of Chemical and Biomolecular Engineering, North Carolina State University, Engineering Building I, 911 Partners Way, Raleigh, NC 27695, (3) ProMetic Life Sciences, 8168 chemin Montview, Mont-Royal, QC H4P 2L7, Canada, (4) Medical Research Service 151, Veterans Affairs Medical Center, Baltimore, MD 21201, (5) Prolias, Laytonsville, (6) Department of Neurology, University of Maryland at Baltimore, 10 N. Greene Street, Baltimore, MD, MD 21201
The potential presence of disease-associated prion proteins in blood and its derivatives poses a challenge for the blood transfusion industry. Prion inactivation has been suggested, but not reduced to practice while removal strategies focused on blood, such as leukodepletion reduce infectivity by less than one log. The use of affinity resins to capture specific targets from a complex medium is a viable complement for leukodepletion. In order to overcome the difficulties of passing a particulated material (red blood cell concentrate) through a packed-bed column, a device containing multiple layers of particle-impregnated membranes was developed. Its performance was evaluated in regards to prion capture, blood quality, and removal of other proteins, among other parameters, with very good results. A comparison with resin in column format showed similar capacities and dissociation constants, indicating that the trapped resin particles are still accessible to the target material.
 

Industrial and Engineering Chemistry Divisional Fellow Award Symposium - Dr. Ruben Carbonell
1:30 PM-4:25 PM, Tuesday, April 8, 2008 Morial Convention Center -- Rm. 231, Oral

Division of Industrial & Engineering Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008