Properties of affinity membranes for h-IgG purification

I&EC 46

Cristiana Boi1, Simone Dimartino1, Ruben G Carbonell, ruben@ncsu.edu2, and Giulio C. Sarti, giulio.sarti@mail.ing.unibo.it1. (1) Department of Chemical Engineering, Mining and Environmental Technologies, Università di Bologna, viale Risorgimento 2, Bologna, 40136, Italy, (2) Department of Chemical and Biomolecular Engineering, North Carolina State University, P.O. Box 7905, Raleigh, NC 27695
Different affinity membranes have been tested for the capture step of monoclonal antibody production. The study has been performed using affinity membranes specifically obtained using cellulose based as well as polyethersulfone based substrates, modified by using different ligands, including protein A mimetic ligands and a specific sequence of six peptides recently selected by in NCSU laboratories; a new protein A membrane as well as a commercially available protein A membrane were also considered. The membranes have been characterized and compared in terms of their ligand densities, binding capacities, ligand utilization and selectivity for the target antibody. Breakthrough performances have been evaluated together with recovery and concentration factors for the most promising membranes. Model simulations of the affinity cycle is also considered, including convection, diffusion and multi-component adsorption to simulate the adsorption, washing and elution steps. Results of the simulation have been compared with the experimental data, giving a good description of the affinity cycle.
 

Industrial and Engineering Chemistry Divisional Fellow Award Symposium - Dr. Ruben Carbonell
1:30 PM-4:50 PM, Monday, April 7, 2008 Morial Convention Center -- Rm. 231, Oral

Division of Industrial & Engineering Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008