Extraction, quantification, and qualification of ribonucleic acid

CHED 843

Thea Wilkins1, Magdy Alabady1, Whitney S. Brewton2, Chase B. Head3, and Corina I. Gonzales3. (1) Dept. Plant and Soil Science, Texas Tech University, Lubbock, TX 79409, (2) Department of Biology - Texas Tech University, Texas Tech University, 5710 4th Street #1801, Lubbock, TX 79416, (3) Texas Tech University
Modern biological research relies on the genetic material to understand the mechanisms of development and organization. RNA is one of the genetic materials that is widely used in many research themes. The quality of RNA is considered a very critical aspect that is taken into account at the beginning of any genetic experiment. In our program, we profile gene expression during the development of cotton fiber cells. RNA accumulation is the targeted measurement. The methodology of RNA extraction and qualification is essential for successful experiments. We use a specific method to extract high-quality RNA from cells. In conclusion, by using our RNA extraction method followed by quality control measurements using Nano-drop and Bio-analyzer instruments we were able to produce high-quality RNA, which has been successfully used in several applications including PCR, cDNA, microarray, and in libraries.
 

Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster

Division of Chemical Education

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008