CHED 284 |
| The Bacterial Kidney Disease (BKD) is caused by Renibacterium salmoninarum, a gram-positive diplobacillus that produces chronic, systemic infection characterized by granulomatous lesions in the kidney and other organs. We are developing a nonlethal screening detection method based on near infrared spectroscopy. Near infrared spectra were acquired on morts and anesthetized fish with a fiber optic probe applied directly to skin and scales. The spectroscopic information was correlated to the fish BKD status, as determined by visual examination of gross kidney morphology, direct fluorescent antibody test, and quantitative polymerase chain reaction test. Categorization of fish according to their health status utilized Principal Component Analysis and Soft Independent Modeling of Class Analogy. These data support our hypothesis that specific changes in body composition between healthy fish and those with BKD may allow for development of a nonlethal screening method that recognizes presence of BKD in salmonids before development of external clinical signs. |
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Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster
Division of Chemical Education |