Determination of L-fucose concentration in intestinal mucus of CD-1 mice

CHED 254

Drew A. Crawford, dcrawford07@student.sosu.edu1, G. Aaron Hightower, jesusdrumma@aol.com1, Andrew Fabich, afabich@ou.edu2, Tyrrell Conway, tconway@ou.edu3, Paul Cohen4, and Joel T. Smith, tsmith@sosu.edu5. (1) Department of Chemistry, Southeastern Oklahoma State University, 1405 N. 4th, c/o Dr. Tim Smith - PO Box 4025, Durant, OK 74701, (2) Department of Microbiology, University of Oklahoma, Norman, OK, (3) Botany and Microbiology, University of Oklahoma, Norman, OK 73019, (4) Microbiology, University of Rhode Island, (5) Department of Chemistry, Computer and Physical Sciences, Southeastern Oklahoma State University, 1405 N. 4th Ave., Durant, OK 74701
Escherichia coli (E. coli) is perhaps the most studied of all model organisms. Pathogenic strains, such as E. coli O157:H7, pose a significant risk to health. Recent findings using whole-genome expression profiling revealed what E. coli MG1655 genes were induced by the nutrients available in the mammalian intestine. The mutational analysis/microarray study found that only mutations in sugar pathways affected the colonization of the bacteria. Fucose is believed to be a key signaling sugar in the uptake of sugars in the E. coli environment which is the intestinal mucus membrane of the host. The concentration of fucose in the intestinal mucus of streptomycin treated CD-1 mice was determined by two complementary analytical methods, capillary electrophoresis-laser induced fluorescence and capillary electrophoresis-mass spectrometer. The mucus represents a very complex matrix. Prior to analysis, sugars were tagged with the fluorophore, 2-aminoacridone. The method of standard additions was used to account for matrix effects.
 

Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster

Division of Chemical Education

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008