CHED 313 |
| Here in, we expand on a simple and effective method for detection of DNA using unmodified gold nanoparticles that was first researched by Huixiang Li and Lewis Rothberg in 2004. Other methods had already been developed to detect DNA with nanoparticles, but those methods are more expensive and more complicated. The development of the technique by Li and Rothberg have made detecting DNA with nanoparticles something smaller undergraduate universities can do because the technique is inexpensive and requires no modification of chemicals like other previous detection methods used. The aim of our study was to test the robustness of this method in the presence of a number of different biological molecules. In our study, we varied the concentration of the amino acids L-Leucine (0.1 g/100ml & 0.5 g/100ml) and L-Aspartic acid (0.1 g/100ml & 0.5 g/100ml). We also varied the concentration of Bovine Serum Albumin (0.02 g/ml & 0.1 g/ml), and lastly we varied the concentration of the enzyme Trypsin (0.1 g/ml & 0.5 g/ml). Our results indicate that this system can detect DNA on orders down to 5 nmol while in the presence of the above mentioned biological molecules. |
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Undergraduate Research Poster Session: Nanotechnology
11:00 AM-1:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster
Division of Chemical Education |