CHED 773 |
| LC/MS, an up and coming technique for forensics, has been making steady inroads into laboratories over the past decade. Adoption of LC/MS has taken much longer in forensics due to cost. Consequently, the legal system has been slow to accept these methods as valid and admissible. With the eventual increased acceptance of LC/MS comes a need for improved methods and sample prep development. The goal is to get LC/MS to the point where it's as information-dense as its GC/MS counterpart. LC/MS is applicable to more sample types, and has greater sensitivity, than GC/MS. Our ultimate goal is to improve separation and analysis protocols for fragmented DNA by LC/MS/MS. Dyes can be problematic and costly in LC applications. Most dyes utilized to visualize DNA are suspected carcinogens. Non-carcinogenic dyes are often difficult to elute from agarose gels and/or result in insufficient separation from purified DNA and hampered by low detection sensitivities. The results of a study with the aim of improving visual band detection while at the same time improving DNA fragment elution from agarose gels will be presented |
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Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster
Division of Chemical Education |