pH Values of the zwitterionic buffer AMPSO from 5 to 55°C

CHED 295

Jared L. Durden, jdurden@drury.edu, Toshi Ghosh, tghosh@drury.edu, Ho Dang, Blake M. Bodendorfer, Lakshmi N. Roy, lroy@drury.edu, and Rabindra N. Roy, rroy@drury.edu. Walter H. Hoffman Department of Chemistry, Drury University, 900 N. Benton Ave., Springfield, MO 65802
The control of acidity in the biological pH range 6.5 to 7.5 is important in biomedicine. The buffer compound AMPSO has been studied. The pH values for four buffer solutions, for example, 0.08 molal Buffer + 0.08 molal sodium Bufferate + 0.08 molal NaCl; at temperatures 5, 25, 37, 45, and 55°C have been calculated. The cells were used without liquid junction of the type: Pt; H2 (g, 1 atm) | Buffer (m1) + NaBufferate (m2) + NaCl (m3) | AgCl, Ag. The pH at 37°C for AMPSO closely matches the pH of blood. The results will be explained in terms of using it as a primary standard for biological specimens.