Analyzing everyday samples in biochemistry experiments

CHED 778

Tamra O. Shelly, toshelly@marauder.millersville.edu and Aimee L. Miller, aimee.miller@millersville.edu. Department of Chemistry, Millersville University, P.O. Box 1002, Millersville, PA 17551
Students at all levels connect to experiments that involve common samples. We are exploring options for expanding published protocols to include a wider range of experimental samples for use in biochemistry courses. Lipid extraction and cholesterol analysis from eggs and walnuts has been described. We have explored similar analysis of isolates from common foods, including a variety of both plant and animal sources. Analysis of biochemical molecules using GC/MS is an important skill for biochemistry students. By adapting a GC/MS protocol for quantifying cocaine residue on paper currency, we hope to appeal to students interested in the forensics field. We have also used a protocol designed for sports drink analysis to do the same taurine analysis of vitamin-enhanced water. We plan to adapt this procedure to possibly analyze other amino acids being used as additives in products marketed as healthy.