Activation of the silk surface to attach cell adhesion molecules

CHED 1143

Jacqueline M. Latina, jlatina@Princeton.edu1, Jeffrey Schwartz, jschwartz@princeton.edu1, and Thomas J. Dennes III2. (1) Department of Chemistry, Princeton University, Frick Laboratory 261, Washington Road and William Street, Princeton, NJ 08544, (2) Department of Chemistry, Millersville University, PO Box 1002, 1 South George Street, Millersville, PA 17551
Zirconium tetra(tert-butoxide) reacts with surface amide groups of Bombyx mori silk to give (η2-amidate)zirconium complexes in high yield. These surface complexes react to bond the cell-adhesive peptide arginine-glycine-aspartic acid (RGD) to the silk surface. A surface loading of approximately 0.10 nmol/cm2 of RGD is achieved, which is ca. 5 times higher than previously reported attainable on silk by other strategies. Surface coverage of the silk by RGD and the activity of this treated silk for cell adhesion and spreading in vitro are presented.
 

Undergraduate Research Poster Session: Inorganic Chemistry
2:00 PM-4:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster

Division of Chemical Education

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008