CHED 847 |
| The hydroxyl radical-scavenging activity and the inhibition of the generation of superoxide radical anion were evaluated for Costus spp., Rhoeo spathacea, Tapeinochilus ananassae, and Syzygium jambos methanolic and aqueous extracts. These plants have been reported by ethnobotanical practitioners as traditional remedies for the treatment of diabetes. The superoxide radical anion was generated using the Xanthine/Xanthine oxidase system and the decline of absorbance was measured at 560 nm. The hydroxyl radicals were generated by the Fenton reaction. Quercetin was used as reference in both assays. The inhibition of superoxide radical anion of the extracts followed a similar order at 100µg/mL : S. jambos > T. ananassae > Costus spp. > R. spathacea. Costus spp. methanolic extract inhibited hydroxyl-radical deoxyribose cleavage in a concentration-dependent manner at concentrations lower than 100µg/mL. At higher concentrations the extract induce color development. These results showed that methanolic extracts of traditional remedies act as reactive oxygen species scavengers. |
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Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster
Division of Chemical Education |