Synthesis of the capture ligand ethyl (4-nitrophenyl)[11-([1,3-diazole]carbamate)undecyl]phosphonate

CHED 479

Aaron B. Thomas, dstallings@georgiasouthern.edu and Dontarie M. Stallings. Department of Chemistry, Georgia Southern University, Post Office Box 8064, Stateboro, GA 30460
A capture ligand, Ethyl (4-nitrophenyl)[11-([1,3-diazole]carbamate)undecyl]phosphonate, with protein immobilization significance was synthesized. The terminal alcohol of 11-Bromoundecanol was replaced with a protecting group, trimethylsilyl. (11-Bromoundecyl)oxy(trimethyl)silane was phosphonated via a Michaelis-Arbuzov reaction. The protecting group was removed and the terminal hydroxyl group was recaptured. Diethyl (11-Hydroxyundecyl)phosphonate was esterified upon addition of carbonyldiimidazole. 4-nitrophenol was added to Diethyl [11-([1,3-diazole]carbamate)undecyl]phosphonate in order to produce the target molecule, Ethyl (4-nitrophenyl)[11-([1,3-diazole)carbamate)undecyl]phosphonate. Preliminary structural analysis was performed using IR Spectroscopy. All structures were verified upon synthesis via 1H NMR, 13C NMR, DEP-90 NMR and DEP-135 NMR.