Using NMR spectroscopy to study temperature dependent hydrogen bonding in N-methylformamide

CHED 290

Kyung mee Lee, leex2208@morris.umn.edu, Department of Chemistry, University of Minnesota - Morris, 600 E 4th Street, Morris, MN 56267 and Jennifer A. Goodnough, roppj@morris.umn.edu, Chemistry Discipline, University of Minnesota, Morris, 600 E 4th Street, Morris, MN 56267.
We performed experimental nuclear magnetic resonance spectroscopy (NMR) to explore the hydrogen bonding groups in N-Methylformamide (NMF) at varying temperatures. Studying hydrogen bonding groups in NMF that are similar to those in protein provides insights into how temperature changes affect the structure of the macromolecules. NMR provides the information related to the hydrogen bond strength of NMF via chemical shift data. Therefore, the chemical shifts of hydrogen bonding group—NH—in NMF were studied from 278 K to 323K. The relaxation times were measured for the amide deuterium on NMF. Relaxation times can lead to the rotational correlation times via calculation. The correlation time tells us approximately how fast the molecule moves at different temperatures. We observed that the hydrogen bond strength decreases as temperature increases. Thus, we report the relaxation time and rotational correlation time as functions of temperature for NMF.
 

Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster

Division of Chemical Education

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008