Determination of 2-mercaptobenzothiazole in rubber gloves and elastic wraps

CHED 234

Ryoko H. Vomsaal, VomsaalR@bucmail.barry.edu1, Miesty P. Woodburn, mwoodbur@fau.edu2, Cyril Párkányi, parkanyi@fau.edu2, and Zuzana Zajickova, zzajicko@fau.edu1. (1) Department of Physical Science, Barry University, 11300 NE 2nd Ave, Miami Shores, FL 33161, (2) Department of Chemistry and Biochemistry, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431
An allergenic substance present in rubber products, 2-mercaptobenzothiazole (MBT) was analyzed by recently developed high performance liquid chromatography (HPLC) method. Commercially available rubber gloves and elastic wraps were selected for the extraction. Optimization of the extraction conditions was explored using several solvents with different properties. Dichloromethane was found to be the most suitable solvent. In addition, samples were immersed in artificial sweat, further extracted with dichloromethane, and dried over anhydrous sodium sulfate. Extracts were evaporated to dryness under a stream of nitrogen, redissolved in acetonitrile, and injected into a Hypersil ODS column. MBT was eluted isocratically with MeCN:H2O (6:4). An alternative gradient method was developed as well. 2-Hydroxybenzothiazole was used as an internal standard. Detection was performed at a single wavelength of 325 nm.
 

Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster

Division of Chemical Education

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008