CHED 833 |
| This project focuses on the use of sequence and ligation independent cloning (SLIC) to generate plasmids containing the Vibrio harveyi lux gene system. These plasmids will then be used to transform plants so that transgenic plants will be able to express the lux gene system. The lux system is composed of four components the first of which is a luciferase that generates light using a fatty aldehyde substrate. The second, third and fourth are a reductase, transferase, and synthetase which are responsible for generating the fatty aldehyde from a carboxylic acid. The novel aspect of this project is the use of the SLIC system to generate the plasmids without the need for more than one restriction digest. The luciferase luxAB fusion gene has already been cloned into a plasmid and current work involves the making of the reductase/transferase plasmid as well as the synthetase/FMN reductase plasmid. |
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Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster
Division of Chemical Education |