CHED 1622 |
| Nα-benzoyl-arginine-p-nitroanilide (BAPNA) is often used as a substrate for Serine and Cysteine proteases experiments. Hoverer, BAPNA is not water soluble, thus, a polar aprotic solvent, such as dimethyl sulfoxide (DMSO) must be used throughout the experiment. Unfortunately, DMSO is not one of the desirable solvents for undergraduate Biochemistry laboratory. This year students performed their experiments with virtual analytical instrument to observe the enzyme activity. A virtual analytical instrument is a simulated instrument that shows the same control interface as of the actual instrument. The virtual instrument can also provide “a simulated run” for different samples and instrument parameters. Using the virtual analytical instrument, students can perform their experiments without physical instrument and reagents. In this presentation, the implementation of the virtual analytical instrument to enzyme kinetics experiment for Biochemistry laboratory is discussed. A group of students also performed the actual experiments using the physical instruments and reagents. Comparison of their performance and mastery-level of the subject will also be presented. |
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General Papers
1:00 PM-3:20 PM, Thursday, April 10, 2008 Hilton New Orleans Riverside -- Jefferson Ballroom, Oral
Division of Chemical Education |