Effect of high concentration collagen type I on matrix induced glioma expansion and migration

CHED 978

Christian Gluck, cbg2103@columbia.edu, Cheng Guo, cg2165@columbia.edu, and Laura J. Kaufman, kaufman@chem.columbia.edu. Department of Chemistry, Columbia University, 3000 Broadway, New York, NY 10027
We study the invasiveness and motility of glioblastoma multiforme in three dimensional (3D) collagen type I matrices of high concentration using both single cell and glioma spheroid in vitro assays. After stably transfecting rat C6 glioma cells with plasmids expressing pEGFP-actin, we show the effects of incubating these cells in such collagen networks via fluorescent microscopy and confocal reflectance microscopy. Initial results show that at higher concentrations, from 5 to 10 mg/ml collagen, glioma cell invasiveness is reduced along with the extent of matrix reorganization. To further analyze this aspect of glioma behavior in thick collagen matrices, we use several drugs to investigate the signaling molecules involved in this phenomenon. Initial results from inhibitor assays combined with statistical analyses of glioma migration in collagen gels that vary in collagen concentration, pore size, and stiffness suggest that saltatory migration of glioma cells in vitro may be dependent on myosin-II function.