Validation of a high pressure liquid chromatography assay for the quantization of the DB-67 camptothecin analog in human whole blood

CHED 242

Brent Allen Roach, broach@transy.edu, Deaprment of Chemistry, Transylvania University, 300 North Broadway, Lexington, KY 40508 and Eva Csuhai, ecsuhai@transy.edu, Division of Natural Sciences and Mathematics, Transylvania University, 300 North Broadway, Lexington, KY 40508.
Camptothecins are a class of chemotherapeutic agents that act by binding and stabilizing the DNA/DNA I Topoisomerase I complex. Upon this stabilization, DNA becomes excessively strained during replication, resulting in single and double stranded breaks which eventually results in cell death. The ultimate goal of camptothecin analog drugs, in particular DB-67 (third generation Camptothecin analog), is tumor regression dependent upon cancer cell populations continuously being exposed to the closed ring, lactone form of the drug. Assay development for the quantization of DB-67 in human blood is necessary in order to determine the levels of active drug (lactone) to inactive drug (carboxylate). Previous assays focused upon quantization in human and mouse plasma, ignoring red blood cell sequestration of the hydrophobic lactone form. HPLC analysis is a necessary analytical technique in determining drug levels, and is a stable and efficient method through which drug levels in whole human blood can be idealized.
 

Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster

Division of Chemical Education

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008