CHED 244 |
| The development of Two-Dimensional Liquid Chromatography has been limited by the paucity of pairs of stationary phases with orthogonal chromatographic selectivities. This research focused on characterizing the selectivity of a carbon-clad zirconia stationary phase with very high coverage (> 99%) of the zirconia surface. Using the Hydrophobic Subtraction Model of reversed-phase HPLC column selectivity developed by Snyder, the contributions of hydrophobicity, steric resitance, hydrogen bonding, and Coulombic interaction to retention of low-molecular-weight analytes were estimated. Comparison of these results with Snyder's database of over 350 commercially-available reversed-phases shows that the carbon-clad zirconia phase will provide orthogonal selectivity when paired with many other reversed-phase columns; this cannot be said for most of the other 350 phases. Finally the hydrophobicity and aromaticity of the carbon-clad zirconia phase were characterized using homolog series of nonpolar molecules which provide additional insight into the unique selectivity of this phase compared to other commercially-available reversed-phase materials. |
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Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster
Division of Chemical Education |