Comparison of NMR analysis to HPLC method following the degradation of creatine monohydrate in various formulations

CHED 957

Kiyana Edwards,, Jeavon Inniss,, and Tony Wallner, Department of Physical Sciences, Barry University, 11300 NE 2nd Ave, Miami Shores, FL 33161
Interest in creatine exists due to its popularity as a nutritional supplement for increasing muscle size, endurance, and performance based on numerous studies. NMR (Nuclear Magnetic Resonance) spectroscopy was used to determine the degradation of creatine monohydrate into creatinine. The NMR spectra of various mole ratios of the two compounds were obtained in D2O to generate a calibration curve. The stability of creatine was studied by determining the effects of water and acid (pH = 1), at various contact times. Additionally, the stability of creatine in grape juice was determined. Recent reports have shown an increased efficacy of creatine when combined with beta-alanine. Finally, the stability of creatine combined with beta-alanine was examined over time in the various solvents. Previous analyses have used reverse phase HPLC (High Performance Liquid Chromatography) to study this degradation. Therefore, the NMR results were compared to reverse phase HPLC method. Over a two week period, creatine degraded into creatinine in water and acid (pH= 1). Results from the NMR spectra were consistent with the HPLC data, but were obtained with shorter time.