CHED 830 |
| Amphitrite ornata Dehaloperoxidase (DHP) is a heme-containing enzyme capable of catalyzing the oxidative removal of halogens from organic compounds, a reaction that could be potentially exploited for environmental bioremediation processes. Because the exact mechanism though which DHP acts is currently unresolved, this senior research project seeks to elucidate some of the reactive properties of DHP by developing a heme-protein based model system utilizing a more thoroughly characterized protein, Cytochrome c peroxidase (CCP). Here, the heme active site of CCP will be enlarged on the distal side through the introduction of an R48A mutation using site-directed mutagenesis. The engineered cavity will then be examined for potential binding and catalytic conversion of halogenated organic substrates. Results of this study will contribute to the understanding of DHP activity and possibly illuminate some properties of its catalytic mechanism. |
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Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster
Division of Chemical Education |