Development of molecular colorimetric sensors for Flunitrazepam and other narcotics and the use of circular dichroism to determine absolute configuration of methamphetamine

CHED 265

Katie Wilcox, katie.wilcox@doane.edu and Andrea E. Holmes, andrea.holmes@doane.edu. Department of Chemistry, Doane College, 1014 Boswell Ave, Crete, NE 68333
Our lab is interested in developing aptamer-based colorimetric molecular sensors for flunitrazepam (better known as “roofies”) and other commonly abused narcotics. A combinatorial approach was used with the aim of creating the most effective and inexpensive sensor possible. Various dyes and aptamers were tested at a range of concentrations. The goal was to create a portable, commercially available test kit for detecting the presence of a drug in drinks or for those investigating other drug-related crimes. Circular dichroism spectrometry was used to differentiate between the enantiomers of methamphetamine and other chiral drugs which form a complex between the narcotic and a zinc-porphyrin tweezer. This is important because for many chiral drugs, the two enantiomers have completely different physiological effects. This porphyrin tweezer technology may be used to form a sensor for the different enantiomers of methamphetamine, and possibly, may be used to create a colorimetric sensor for the drug.