Investigating properties of porous stationary phase particles in reversed-phase liquid chromatography

CHED 243

Malori Evans and Carolyn R. Simmons, csimmons@shc.edu. Department of Chemistry, Physics, and Engineering, Spring Hill College, 4000 Dauphin Street, Mobile, AL 36608
Inorganic salts such as NaNO3 have long been used as void volume markers in reversed-phase liquid chromatography. The exclusion of the anions from the stationary phase pores in unbuffered mobile phases has been well documented and is commonly referred to as Donnan exclusion, or electrostatic exclusion. In this study, inorganic salts have been used to explore interesting properties of the porous stationary phases, including mobile phase broadening effects and the apparent existence in some columns of secondary pore networks.