Studies of chicken antigen/antibody interactions with gold and silver nanoparticles

CHED 266

Jarrett N. Proctor, proctor7260@netzero.net1, Robin M. Bright1, and Caryn S. Seney, seney_cs@mercer.edu2. (1) Department of Chemistry, Fort Valley State University, 1005 State University Dr, Fort Valley, GA 31030-4313, (2) Department of Chemistry, Mercer University, 1400 Coleman Avenue, Macon, GA 31207
Gold nanoparticles will be synthesized using documented methods involving the reduction of gold ions by citrate. Characterization of these gold nanoparticles based on size will be determined using UV-Visible spectroscopy and transmission electron microscopy (TEM). Gold nanoparticle preparations with an optical spectrum that results in λmax at approximately 520 nm and a full width at half max less than 90 nm are then used in the conjugation of antibody/gold nanoparticles and antigen/gold nanoparticles. In addition, polydisperse silver nanoparticles will be synthesized using documented methods involving the reduction of silver ions by citrate and borahydride. After characterization of the nanoparticles, chicken antibody/gold nanoparticle and anti-chicken antigen/gold nanoparticle conjugates will be formed, followed by optimization of immunoassay conditions. For analysis of the conjugates, surface-enhanced Raman spectroscopy (SERS) of the individual protein nanoparticle conjugates will be performed initially using the gold nanoparticles and then in conjunction with the silver nanoparticles aggregation.
 

Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster

Division of Chemical Education

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008