Use of double isotopic dilution in the analysis of drugs in postmortem tissues

CHED 221

Omesh Qasba, c08omesh.qasba@usafa.edu1, John S. Wilkes, john.wilkes@usafa.edu1, Joseph A. Levisky, Joseph.Levisky@usafa.af.mil1, and Werner Jenkins, wjnjenkins@comcast.net2. (1) Department of Chemistry, US Air Force Academy, 2355 Fairchild Drive, USAF Academy, CO 80840, (2) Toxicology, El Paso Country Coroner's Office, East Las Vegas St., Colorado Springs, CO 80903
Quantitative analysis for drugs in postmortem tissues continues to challenge analytical toxicologists. Traditionally, an internal standard is added to homogenized tissue and the amount of drug is determined by comparing the drug/internal standard ratio to ratios of known calibration curves. By using different concentrations of two deuterated drug analogs, analysis is accomplished by a single extraction and a single GC/MS injection. Most importantly, the tissue itself becomes the matrix used to establish the calibration curve.
 

Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster

Division of Chemical Education

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008