CHED 248 |
| Anemonefish (Amphiprion ocellaris) represent a major component in the global trade of marine ornamental fish, an industry which nets approximately $7 billion annually. To serve this market, anemonefish are now being bred in captivity and studies have shown that the presence of four polyunsaturated fats Eicosapentanoic (EPA), Arachidonic (AA), Docosapentanoic (DPA) and Docosahexanoic Acids (DHA) are vital to normal growth and development in adult and larval stages. Historically, gas chromatography (GC) has been the most common method for analyzing fatty acids. Recently we have developed a method using liquid chromatography mass spectrometry (LCMS) to analyze the four PUFAs of interest in an ongoing anemonefish breeding project. We will present results comparing LCMS fatty acid analysis to the classic GC/FAMES analysis and expect to show that LCMS is preferable to GC/FAMES because 1) the derivatization steps are simpler 2) chromatographic runs are shorter and 3) detection limits are lower. |
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Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster
Division of Chemical Education |