CHED 770 |
| Activated charcoal is known for its adsorptive properties and is commonly used for filtering, purification and adsorption of organic impurities or contaminants from solution. Although used extensively in hospital emergency rooms to treat poisonings and overdoses, its use to adsorb allergenic proteins has not been studied. This research investigates the factors affecting protein binding to activated charcoal. Various purified proteins, or protein mixtures, were treated with activated charcoal under various conditions. After allowing the protein to bind to the charcoal, the charcoal, along with any bound protein was centrifuged from the solution. Any unbound protein remaining in solution was detected by gel electrophoresis or quantitative protein assay. The binding efficiency, rate of binding, and binding reversibility of protein to activated charcoal will be presented. The effect of binding time, temperature, and charcoal concentration on protein binding will also be shown. |
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Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster
Division of Chemical Education |