Examining population variation in black cohosh chemical constituents using mass spectrometry and induction of differentiation on HL-60 cells

CHED 836

Christa A. Kucharczyk, ckucharczyk0@frostburg.edu1, Janine McKnight1, Andrew Minnick1, Danielle Mortensen2, Peggy Biser, pbiser@frostburg.edu1, and R. Scott Fritz, rfritz@frostburg.edu2. (1) Department of Chemistry, Frostburg State University, Frostburg, MD 21532, (2) Department of Biology, Frostburg State University, Frostburg, MD 21532
Black cohosh is an herbal remedy used by many women to treat menopausal symptoms. An HPLC-MS was used to create a standard curve for each of six chemicals: actein, 27-deoxyactein, cimicifugoside, cimifugin, ferulic acid, and caffeic acid. The roots of various populations of black cohosh were extracted using various solvents and then analyzed using the HPLC-MS for these components. A cell line of HL-60 cells, a human leukemia cell line, was established and then tested for differentiation. The extracts of black cohosh will be tested for their ability to differentiate HL-60 cells.
 

Undergraduate Research Poster Session: Biochemistry
2:00 PM-4:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster

Division of Chemical Education

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008