Aptasensor for protein detection: Optimization of parameters for high performance aptamer sensor

ANYL 150

Balamurugan Subramanian, suba04@lsu.edu, Anne Obubuafo, Robin L. McCarley, tunnel@lsu.edu, Steven A. Soper, and David A Spivak, dspivak@lsu.edu. Department of Chemistry and Center for Biomodular Multiscale Systems, Louisiana State University, 232 Choppin Hall, Baton Rouge, LA 70803
Aptamers are oligonucleotides selected toward a particular target molecule such as protein, drug or a cell, from a large library of oligonucleotides by a combinatorial process called SELEX. These artificial receptors can be used as a molecular recognition unit in a biosensor. We are interested in developing an aptamer based sensor for the detection of proteins and protein biomarkers. In this presentation, we discuss the effect of the linkers (oligo thymidine and oligo ethylene glycol) and the co-adsorbents on aptamer monolayer formation and its effect on protein binding efficiency and specificity. Aptamers binds specifically to protein thrombin was used as the model system. The effect of formation of aptamer monolayer under the flow condition will also be discussed.

 

Nanomaterials in Analytical Chemistry
1:25 PM-5:05 PM, Monday, April 7, 2008 Morial Convention Center -- Rm. 335, Oral

Division of Analytical Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008