Preparation and characterization of covalently linked bacteriophage-nanoparticle networks

ANYL 147

Hongxia Zhang, zhangho@auburn.edu, Department of Chemistry & Biochemistry, Auburn University, 179 Chemisry Building, Auburn University, Auburn, AL 36849 and Curtis Shannon, shanncg@auburn.edu, Department of Chemistry and Biochemistry, Auburn University, Auburn, AL 36849-5312.
Assembly of biological molecules and nanoparticles to generate novel hybrid materials is an active area of investigation with applications ranging from sensor development to the diagnosis and treatment of certain diseases. Typically, the bonding between biomolecules and nanoparticles is based on hydrophobic interactions, van der Walls contact, and/or electrostatic forces. In this contribution, we report on the covalent attachment of filamentous bacteriophage thiolated with 2-iminothiolane to gold nanoparticles. Results from UV-VIS spectroscopy indicate that the reaction between primary amines on phage and 2-iminothiolane takes place very rapidly. TEM and AFM imaging clearly shows that the formation of networks of bacteriophage and nanoparticles has occurred. Surface enhanced Raman spectroscopy was used to investigate nature of the interaction between thiol-functionalized fd bacteriophage and gold nanoparticles. The conjugate network generated in our work has promising applications in biosensor field. Preliminary investigations on the use of these materials to modify electrode surfaces will be discussed.
 

Nanomaterials in Analytical Chemistry
1:25 PM-5:05 PM, Monday, April 7, 2008 Morial Convention Center -- Rm. 335, Oral

Division of Analytical Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008