Label-free attomolar detection of proteins using integrated nanoelectronic and electrokinetic devices

ANYL 138

Jian-Ru Gong, jrgong@cml.harvard.edu1, Wang Lu, luwang@cml.harvard.edu1, Gengfeng Zheng, gzheng@cmliris.harvard.edu2, and Charles M. Lieber, cml@cmliris.harvard.edu2. (1) Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, (2) Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Conant 015, Cambridge, MA 02138
High-sensitivity screening of biomarkers is critical to areas ranging from early disease detection and diagnosis to bioterrorism surveillance. Here we report the development of integrated nanoelectronic and electrokinetic devices for label-free attomolar detection of proteins. Electrically-addressable silicon nanowire field-effect transistors and electrodes for electrokinetic transport were integrated onto a common sensor chip platform, and the nanowire devices were subsequently functionalized with receptors for selective biomarker detection. Nanowire devices modified with monoclonal antibody for prostate specific antigen exhibit up to a 10^4 increase in sensitivity with electrokinetic transport active, while maintaining excellent selectivity. Studies of the frequency, amplitude and solution ionic strength dependence of the enhancement will be described. These studies demonstrate the potential for real-time, label-free detection of proteins with integrated nanoelectronic sensors to the 10-attomolar level, and could open up opportunities in early stage disease detection and the analysis of proteins from single cells.
 

Nanomaterials in Analytical Chemistry
8:25 AM-12:25 PM, Monday, April 7, 2008 Morial Convention Center -- Rm. 335, Oral

Division of Analytical Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008