ENVR 74 |
| Our group uses embryonic zebrafish as a model organism to examine in vivo nanomaterial and biological interactions. To directly address the oxidative potential of the fullerene C60, the effects of light and chemical supplementation and depletion of glutathione (GSH) on C60-induced toxicity were evaluated. Reduced light during C60 exposure significantly decreased mortality and toxicity at 200 and 300 ppb C60. Embryos co-exposed to the glutathione precursor, N-acetylcysteine, reduced mortality and induced malformations. Conversely, co-exposure to the GSH synthesis inhibitors, butathionine sulfoximine and diethyl maleate increased the sensitivity of zebrafish to C60. DNA microarrays were used to examine the effects of C60 on the global gene expression at two time points, 36 and 48 hours post-fertilization (hpf). C60 exposure led to alterations in the expression of several key stress response genes. Collectively, these results support the hypothesis that C60 induces in vivo oxidative stress. |
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Environmental Behavior and Fate of Manufactured Nanomaterials
1:30 PM-5:30 PM, Monday, April 7, 2008 Morial Convention Center -- Rm. 235, Oral
Division of Environmental Chemistry |