Low cytotoxicity and lysosome location of malonic acid fullerene derivatives on rat primary brain capillary endothelial cells

ENVR 57

Fang Lao, laof@nanoctr.cn, Chunying Chen, chenchy@nanoctr.cn, Dong Han, Wei Li, Fang Jiao, Cuicui Ge, Ying Liu, and Yuliang Zhao, zhaoyuliang@ihep.ac.cn. Laboratory for Bio-Environmental Effects of Nanomaterials and Nanosafety (NCNST-IHEP), Institute of High Energy Physics and National Center for Nanoscience & Technology of China, 1st North Street, Zhonguancun, Beijing, 100080, China
Many insights were done to improve drug delivery across the blood brain barrier (BBB) and some groups focus on using nanotechnology. However, the direct interaction of nanoparticles with the cells at BBB remains unclear. In this work, we isolated the primary rat brain capillary endothelial cells (rBCECs) and treated them with a bi-malonic acid fullerene derivative C60(C(COOH)2)2. We used CCK-8 cytotoxicity assay and a confocal fluorescent microscope to estimate the toxicity of this nanoparticle and their localization in the cell. The results demonstrated that the fullerene derivative did not affect the cell growth at the dose up to 100µg/ml. The fluorescent images showed the derivative could really cross the external cellular membrane of rBCECs and localized in the lysosomes but not mitochondrias, indicating that the particles may regulate the cell through lysosomes. However, treatment of C60(C(COOH)2)2 had little influence on the release of the endothelin-1 (ET-1).
 

Environmental Behavior and Fate of Manufactured Nanomaterials
8:30 AM-12:10 PM, Monday, April 7, 2008 Morial Convention Center -- Rm. 235, Oral

Division of Environmental Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008