ANYL 135 |
| We have developed a method for synthesizing SERS-active gold nanoparticles via thiosulfate reduction of aqueous tetrachloroauric acid. Results of nanoparticle characterization via UV-vis, TEM, and DLS will be provided, along with a description of the factors found to be critical in controlling the size, polydispersity, aggregation state, and SERS activity of the nanoparticles. Representative solution SERS and SERS microscopy spectra of a variety of analytes will be presented. Attempts to employ these nanoparticles as novel matrices for MALDI mass spectrometry will also be described. In addition, recent attempts to conjugate siglecs (sialic acid binding Ig-like lectins) to the nanoparticles for use in colorimetric assays for glycoproteins based on the surface plasmon resonance shift that occurs upon nanoparticle aggregation will be described. As part of this effort, we will present AFM images of microscope slides functionalized with antibodies to erythropoietin (EPO), the glycoprotein we hope to detect via the siglec-conjugated nanoparticles. |
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Nanomaterials in Analytical Chemistry
8:25 AM-12:25 PM, Monday, April 7, 2008 Morial Convention Center -- Rm. 335, Oral
Division of Analytical Chemistry |