Immunomagnetic separation (IMS) was coupled with Quantum Dot (QD) labeling for the rapid, sensitive and simultaneous detection of Escherichia coli and Salmonella enteritidis. The target bacteria were recovered from solution by antibody coated paramagnetic beads and sandwich complexes were formed by using secondary antibodies labeled with two different QDs. The fluorescence intensities due to the capturing of  different concentrations of E. coli in the presence of S. enteritidis were measured and a linear correlation was obtained between log E. coli concentration and the intensity with a working range of 4.0ื10² and 4.0ื10⁵ cfu ml^-1. The working range for S. enteritidis was found 5.0ื10² and 5.0ื10⁵ cfu ml^-1 in the presence of E. coli. The selectivity of the developed sensor was examined with different bacteria from coliform group which did not produce any significant response.