Measurement of diffusion coefficients for bacteriophage MS2 in solutions and at solid-liquid interface: A fluorescence correlation spectroscopy study

ENVR 264

Yan Yu, yanyu2@uiuc.edu1, Mo Jiang, mojiang2@uiuc.edu1, Thanh H. Nguyen, thn@uiuc.edu2, and Steve Granick, sgranick@uiuc.edu3. (1) Department of Material Science and Engineering, University of Illinois at Urbana-Champaign, 1304 W Green Street, Urbana, IL 61801, (2) Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, 205 N. Mathews Ave., Urbana, IL 61801, (3) Department of Material Science and Engineering & Center of Advanced Materials for the Purification of Water with Systems, University of Illinois at Urbana-Champaign, 1304 W. Green St., Urbana, IL 61801
Effective control of viral pathogens for water treatment requires a fundamental understanding of virus interactions with each other and with solid surface under environmentally relevant conditions. In this study, we applied fluorescence correlation spectroscopy (FCS) to measure diffusion coefficients of bacteriophage MS2, a model virus, in solutions and at solid-liquid interface. Solution ionic strengths varied from 1 mM to 200 mM and divalent cations Ca2+ or Mg2+ varied from 0 to 1 mM. A clean glass surface and a glass surface covered with natural organic matter were selected as representative environmental hard and soft surfaces. Preliminary results suggest that MS2 bacteriophage has weak but detectable native fluorescence. Current work focuses on aggregation dynamic of MS2 in solutions and on diffusion coefficients of MS2 at solid surfaces as a function of surface coverage
 

Advances in Drinking Water Disinfection and Disinfection Byproduct Management
6:00 PM-8:00 PM, Wednesday, April 9, 2008 Morial Convention Center -- Hall A, Poster

Division of Environmental Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008