Cytotoxicity of nanostructural materials in MLO-Y4 bone cells

PHYS 408

Meena Mahmood, mwMahmood@ualr.edu1, K. Vyas2, L. I. Plotkin2, Enkeleda Dervishi, exdervishi@ualr.edu3, Zhongrui Li, zxli3@ualr.edu4, Mustafa Abd Al-Muhsen1, T. Bellido2, and Alexandru S. Biris, asbiris@ualr.edu4. (1) Nanotechnology Center and Applied Science Department, University of Arkansas at Little Rock, 2801 S. University Ave, Little Rock, AR 72204, (2) Center for Osteoporosis and Metabolic Bone Diseases, University of Arkansas for Medical Sciences, Little Rock, AR 72204, (3) Applied Science dept, Nanotechnology Center, University of Arkansas at Little Rock, 2801 S. University Ave, Little Rock, AR 72204, (4) Applied Science Department, Nanotechnology Center, University of Arkansas at Little Rock, 2801 South University Avenue, Little Rock, AR 72204
The cellular toxicity of three types of nanomaterials, silver (Ag-NPs) and gold nanoparticles (Au-NPs) and single wall carbon nanotubes (CNTs) was studied by cellular uptake of MLO-Y4 bone cell lines pre-treated with apoptotic agents such as dexamethasone, etoposide, Ethanol and DMSO.

Electron and optical microscopy analysis indicated that the dimensions of the living cells treated by Au-NPs increased as compared to that of the cells exposed to Ag-NPs and CNTs.

Statistical analysis showed significantly higher survival rates of the cells exposed to α-Modified Essential Medium only or with apoptotic agents as compared to the cells cultured with combinations of Au-NPs, Ag-NPs and CNTs. This cytotoxicity study revealed that the Au-NPs had a significant lower cytotoxic effect than the Ag-NPs and CNTs, values reflected by the percentage of the dead cells vs. living cells.

The main goal of this study was to determine the cytotoxicity of different nanomaterials that can be used for the treatments of the malignant diseases or for the administration of specific medications by cellular uptake.

 

PHYS Poster Session - Nanostructured Materials and Nanophotonics
7:30 PM-10:00 PM, Wednesday, April 9, 2008 Morial Convention Center -- Hall A, Poster

Division of Physical Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008