PHYS 382 |
| The activation energy for helix formation in poly-L-glutamic acid (polyGlu) was measured at cryogenic temperatures in D2O/glycerol solution. Protonation of polyGlu side chains was accomplished using UV-induced photolysis of o-nitrobenzaldehyde to form o-nitrosobenzoic acid at 150 K. The temperature was then raised to 225 K while monitoring the amide I' region to detect helix formation. The temperature range of amide I' absorption changes is related to the distribution of activation energies required for helix formation. The dependence of the activation energy for the coil-to-helix transition on initial helix content is discussed. |
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PHYS Poster Session - General Experiment
7:30 PM-10:00 PM, Wednesday, April 9, 2008 Morial Convention Center -- Hall A, Poster
Division of Physical Chemistry |