ENVR 107 |
| We linked genomic DNA damage induced by the regulated DBP bromoacetic acid (BAA) with toxicogenomic analysis using a PCR array for 84 genes related to human DNA damage and repair. We used a non-transformed, normal, fetal human small intestinal epithelial cell line (FHs 74 Int) to measure the modulation of gene expression after exposure to BAA. Cells were exposed for 30 min or 4 h. Eleven genes showed altered gene expression after 30 min; 3 were down regulated. After 4 h exposure 13 genes exhibited altered gene expression; 8 were down-regulated. One gene displayed changed expression at both time points. Functions for the genes modified were 9 genes related to DNA double-strand break repair, 7 cell cycle arrest, two mismatch repair, two base excision repair, one nucleotide excision repair, one apoptosis and one other. These data will provide insights into the biological mechanism of BAA toxicity. |
|
Advances in Drinking Water Disinfection and Disinfection Byproduct Management
8:30 AM-12:10 PM, Tuesday, April 8, 2008 Morial Convention Center -- Rm. 236, Oral
Division of Environmental Chemistry |