Cell-mineral interfacial forces in adhesion and membrane self-assembly

COLL 113

Nita Sahai, sahai@geology.wisc.edu1, Timothy A. Oleson, toleson@geology.wisc.edu2, Mark J. Stevens, mjstevens@wisc.edu1, and Julie A. Last, jalast@wisc.edu3. (1) Department of Geology & Geophysics; Department of Chemistry, University of Wisconsin-Madison, 1215 West Dayton Street, Madison, WI 53706, (2) Department of Geology & Geophysics, University of Wisconsin - Madison, 1215 West Dayton St., Madison, WI 53706, (3) Department of Surgical Sciences, University of Wisconsin-Madison, 2015 Lindedn Srive, Madison, 53706
We examined cell surface interactions with oxide minerals using the Jurkat line of T-lymphocytes and cell membrane-forming phospholipids in bulk adsorption isotherms and by Atomic Force Microscopy (AFM). The adhesion strength of Jurkat cells as well as dipalmytoylphosphocholine (DPPC) adsorption increases as quartz (α-SiO2) < rutile (α-TiO2) < corundum (α-Al2O3). This trend is consistent with increasing point of zero charge of the oxides, which depends on oxide crystal chemistry and interfacial solvation. Thus, Jurkat cell adhesion and phospholipid adsorption are driven mainly by electrostatic, H-bonding forces, and van der-Waals forces. These results have potential implications for the cellularization (“encapsulation”) event in the early evolution of life, for designing supported lipid bilayers as components of biosensors, coating oxide prosthetic implants with biocompatible membranes and understanding the fate of inhaled dust particles in the lung.
 

The Physical Chemistry of Environmental Interfaces
2:00 PM-5:20 PM, Monday, April 7, 2008 Morial Convention Center -- Rm. 225, Oral

Division of Colloid & Surface Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008