Evaluation of reversed-phase HPLC columns packed with sub-3µm particles

CHED 238

Lisandra Santiago-Capeles, lisandra.santiago@gmail.com1, Richard W. Brice, rwbrice@buffalo.edu2, and Luis A. Colón, lacolon@buffalo.edu2. (1) Department of Chemistry, University of Puerto Rico at Cayey, 205 Antonio R. Barceló Ave., Cayey, PR 00736, (2) Department of Chemistry, University at Buffalo, The State University of New York, Natural Science Complex, Buffalo, NY 14260-3000
Two new and recently introduced HPLC columns were evaluated and compared in terms of efficiency, analysis time, methylene selectivity, mass loadability, and silanol activity. The packed columns had been packed with reversed-phase packing material on sub-3 µm diameter particles (i.e., C18 Luna® 2.5 µm from Phenomenex and C18 HaloTM 2.7 µm from Advanced Materials Technology). The studies were performed using a buffered mobile phase under isocratic conditions. The results show that both columns have comparable performance in terms of efficiency, loadability tolerance, and methylene selectivity. Faster analysis times per unit column length were achieved using the HaloTM column rather than the Luna® column at near the optimum flow rate 0.2 mL/min (~3,500 psi). However, asymmetry factors obtained for amitriptyline were higher in the HaloTM column, which is indicative of a stronger silanol activity. In general, the two columns studied can provide the benefits offered by small particles in HPLC while operating at commonly available system pressures.
 

Undergraduate Research Poster Session: Analytical Chemistry
11:00 AM-1:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster

Division of Chemical Education

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008