Preparation of streptavidin coated cold magnetic nanoparticles using cysteamine

ANYL 137

Hongna Liu, liuhongnaqq@163.com1, Song Li, solisong@163.com2, Nongyue He, nyhe1958@163.com2, and Yan Deng, dy_dengyan@yahoo.com.cn2. (1) State Key Laboratory of Bioeclectonics, Southeast University, Si Pai Lou 2, 210096 Nanjing, China, (2) State Key Laboratory of Bioelectronics, Southeast University, Si Pai Lou 2, 210096 Nanjing, China
A novel method for streptavidin coated GoldMagnetic nanoparticles (SA GoldMagnetic NPs) preparation was reported using cysteamine by covalent binding. Gold-coated iron core-shell structure nanoparticles (Fe@Au) were synthesized and used as carriers of streptavidin. SA GoldMagnetic NPs prepared by covalent interaction (cvSA GoldMagnetic NPs) were treated with cysteamine and glutaraldehyde sequentially. Streptavidin was successfully immobilized onto GoldMagnetic NPs surface by covalent interaction between the aldehyde groups on GoldMagnetic nanoparticles surface and streptavidin. For comparison, SA GoldMagnetic NPs by the methods of physical adsorption (paSA GoldMagnetic NPs) were also prepared. Coupling capacity for streptavidin and stability of these two kinds of GoldMagnetic NPs were evaluated. The paSA GoldMagnetic NPs possess high streptavidin binding capacity and simple preparation procedure. Compared with the physical adsorption, the covalent immobilization capacity of streptavidin onto GoldMagNPs were more stable and would offer more opportunities toward using magnetic composite nanoparticles for biomedical purposes.
 

Nanomaterials in Analytical Chemistry
8:25 AM-12:25 PM, Monday, April 7, 2008 Morial Convention Center -- Rm. 335, Oral

Division of Analytical Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008