DMPC and dendrimer aggregates examined by ITC and DLS

POLY 118

Meghan G. Liroff, mliroff@umich.edu1, Christopher V Kelly, cvkelly@umich.edu2, Pascale R. Leroueil, lerouepr@umich.edu1, Douglas G. Mullen, dmullen@umich.edu3, James R. Baker Jr.4, Bradford G. Orr, orr@umich.edu2, and Mark M. Banaszak Holl, mbanasza@umich.edu1. (1) Department of Chemistry, University of Michigan, 930 N. University, Ann Arbor, MI 48109, (2) Department of Physics, University of Michigan, Ann Arbor, MI 48109, (3) Department of Macromolecular Science and Engineering, University of Michigan, 1500 Chemistry, 930 N. University, Ann Arbor, MI 48103, (4) Center for Biologic Nanotechnology, Department of Biomedical Engineering, University of Michigan, 9220 MSRB III, Box 0648, Ann Arbor, MI 48109
The enthalpies of binding between 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and poly(amidoamine) (PAMAM) dendrimers of various generations will be presented using data obtained via isothermal titration calorimetry (ITC). The interactions were examined in various pH levels and buffer environments. Dynamic Light Scattering (DLS) was used to determine the supramolecular size of the DMPC lipid and PAMAM dendrimer complex. This exploration into the binding of dendrimers and lipids, as well as the determination of size and structure, provides relevant information as to the mechanism by which dendrimers are able to deliver DNA in cell transfection processes.
 

Undergraduate Research in Polymer Science
6:00 PM-8:00 PM, Sunday, April 6, 2008 Morial Convention Center -- Hall A, Poster

Division of Polymer Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008