Single particle tracking studies of G protein-coupled receptors

PHYS 550

James Joseph Drinane, jdrinane@nmu.edu1, George B Barisas2, Deborah A Roess, daroess@lamar.colostate.edu3, Shaorui Xu2, Amber Wolf3, and Peter Winter3. (1) Department of Chemistry, Northern Michigan University, 222 W. Michigan St., marquette, MI 49855, (2) Department of Chemistry, Colorado State University, CO, (3) Department of Biomedical Sciences, Colorado State University, Campus Mail 1872, Fort Collins, CO 80523
G protein-coupled cell membrane receptors are targets of approximately 60-65% of all current pharmaceutical agents in development. Thus, elucidating how these receptors work at the molecular level is crucial to continue to develop new, novel pharmaceutics. Single Particle Tracking (SPT) studies of this type cell membrane receptor will allow such an understanding to be gained by examining the effect that the local membrane environment exerts on cell membrane surface receptors, e.g. Luteinizing Hormone Receptor. SPT is a technique that allows one to determine in vivo a molecule's position as a function of time on the surface of a cell membrane using antibody-conjugated 40 nm gold beads bound to the desired molecule. All measurements were performed via optical light microscopy. Following subsequent data analysis, SPT can provide information about the lateral diffusive behavior observed, and allow one to infer physical interactions between the cell membrane receptor and other physical elements of the cell membrane during.

This work was supported by NSF-REU grant EEC-0649263 and by NIH award RR023156

 

PHYS Poster Session - General Theory
7:30 PM-10:00 PM, Wednesday, April 9, 2008 Morial Convention Center -- Hall A, Poster

Division of Physical Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008