ANYL 175 |
| This report describes the assembly of laterally diffusive lipid layers within the pores of colloidal crystals for potential applications in membrane based sensing. Encapsulation within colloidal crystals was monitored by fluorescence microscopy and showed an increase of surface associated lipid by a factor of 15 to 73 depending upon the method used to introduce the lipid to the crystalline substrate. Protein adsorption studies suggest that the crystal pores are not obstructed, and the silica surface of the crystal is passivated with respect to adsorption of a model protein when coated with a lipid bilayer. Furthermore, the mesoporous environment of the colloidal crystal is found to protect lipid films from drying and rehydration processes that destroy planar supported lipid bilayers. The potential of colloidal crystal encapsulated lipid films for chemical sensing is demonstrated by a model protein binding assay that utilizes lipid films doped with biotinylated lipid and fluorescently labeled avidin. |
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Directed Assemblies Using Surface Templates
8:25 AM-12:05 PM, Wednesday, April 9, 2008 Morial Convention Center -- Rm. 335, Oral
Division of Analytical Chemistry |