CHED 1330 |
| A major cause of heart failure in developed countries is myocardial infarction. Recently, the use of embryonic stem cells (ESCs) has been proposed as a potential therapy for the treatment of this disorder. Typically, ESCs are delivered by direct injection into the myocardium; direct injection results in low engraftment. We have developed a mouse ESC embedded small intestinal submucosa (SIS) sheet which could be transplanted directly onto the surface of the heart and would allow for localized and thus higher engraftment of stem cells. Mouse ESCs (with or without Leukemia Inhibitory Factor or LIF) were cultured with SIS sheets using three conditions: pretreatment with mouse ESCs under static culture for 72 hours then shaker seeded for 72 hours; pretreatment with mouse ESCs under static culture for 72 hours without shaker seeding; and shaker seeded for 72 hours without pretreatment. Cells were isolated from sheets and counted to assess efficiency and proliferation. When cells were shaker seeded, the amount of cells embedded within sheets increased at least 31x compared to no shaker seeding. Viability also increased with shaker seeding compared to static culture. These findings illustrate: (a) that we can successfully develop an SIS sheet embedded with mouse ESCs using shaker seeding and (b) the issues that need to be addressed when making cellular constructs. |
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Undergraduate Research Poster Session: Medicinal Chemistry
2:00 PM-4:00 PM, Monday, April 7, 2008 Morial Convention Center -- Hall A, Poster
Division of Chemical Education |